Jaka Kragelj

Jaka_Kragelj

Jaka Kragelj graduated in Biochemistry at the Faculty of Chemistry and Chemical Technology, University of Ljubljana. In his master thesis, he studied the molecular mechanism of phospholipase A2 enzymes by identifying novel phoshpholipase A2 interaction partners. He acquired additional practical skills during an internship in the structural biology laboratory at NorStruct, University of Tromsø. He first worked with IDPs and became interested in this field during an internship in the Lemke group at EMBL, Heidelberg.

 

Researcher
Jaka Kragelj
Project Topic
Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway.
Project Description
Mitogen-activated protein kinase (MAPK) cell signalling pathways feature three sequentially acting protein kinases making up a signalling module: an MKKK (MAPK kinase kinase) that phosphorylates and thereby activates an MKK (MAPK kinase), which then activates the MAPK by phosphorylation. My work focuses on the c-Jun N-terminal kinase (JNK) signalling pathway. While the structures of the catalytic domains of the involved kinases are known, the N-terminal intrinsically disordered regulatory domains pose a bigger challenge because of their flexibility and therefore call for NMR. We aim at understanding the molecular basis for signalling specificity via structural characterisation. We use NMR spectroscopy together with methods for conformational ensemble selection to characterise these disordered proteins and their complexes. New methods or extensions to the already existing computational methodology are being developed where the projects needs require it.
Project Home
CNRS-IBS
Supervisor
Martin Blackledge
 Starting Date September 2011
Publications

Kragelj J, Ozenne V, Blackledge M, Jensen MR. Conformational propensities of intrinsically disordered proteins from NMR chemical shifts. Chemphyschem. 2013;14(13):3034-45.

Meetings


XIVe Journée Rhône-Alpes de RMN
Poster: "Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway"
May 2013 - Lyon, France

25th ICMRBS 
Poster: "Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway"
August 2012 - Lyon, France 2012

XIIIe Journée Rhône-Alpes de NMR
Poster: "Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway"
June 2012 -Rhône-Alpes, France 

Intensive Training Module – Marie Curie Training Network
Protein Structure Analysis using NMR Chemical Shifts
May 2012 -Cambridge, United Kingdom


Complementary Skills Training – Marie Curie Training Network
Self Marketing, Project Management, Benchmarks and Competition in Research
February 2012 -St. Moritz, Switzerland


Intensive Training Course 2011 – Marie Curie Training Network

Bioinformatics and Structural biology of IDPs
October 2011 -Budapest, Hungary

 

Scientific Posters:


 jaka_posterjpg

Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway
XIIIe Journée Rhône-Alpes de RMN, Grenoble, France
June 2012