Jaka Kragelj
Jaka Kragelj graduated in Biochemistry at the Faculty of Chemistry and Chemical Technology, University of Ljubljana. In his master thesis, he studied the molecular mechanism of phospholipase A2 enzymes by identifying novel phoshpholipase A2 interaction partners. He acquired additional practical skills during an internship in the structural biology laboratory at NorStruct, University of Tromsø. He first worked with IDPs and became interested in this field during an internship in the Lemke group at EMBL, Heidelberg.
Researcher |
Jaka Kragelj |
Project Topic |
Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway. |
Project Description |
Mitogen-activated protein kinase (MAPK) cell signalling pathways feature three sequentially acting protein kinases making up a signalling module: an MKKK (MAPK kinase kinase) that phosphorylates and thereby activates an MKK (MAPK kinase), which then activates the MAPK by phosphorylation. My work focuses on the c-Jun N-terminal kinase (JNK) signalling pathway. While the structures of the catalytic domains of the involved kinases are known, the N-terminal intrinsically disordered regulatory domains pose a bigger challenge because of their flexibility and therefore call for NMR. We aim at understanding the molecular basis for signalling specificity via structural characterisation. We use NMR spectroscopy together with methods for conformational ensemble selection to characterise these disordered proteins and their complexes. New methods or extensions to the already existing computational methodology are being developed where the projects needs require it. |
Project Home |
CNRS-IBS |
Supervisor | Martin Blackledge |
Starting Date | September 2011 |
Publications |
Kragelj J, Ozenne V, Blackledge M, Jensen MR. Conformational propensities of intrinsically disordered proteins from NMR chemical shifts. Chemphyschem. 2013;14(13):3034-45. |
Meetings |
25th ICMRBS XIIIe Journée Rhône-Alpes de NMR Intensive Training Module – Marie Curie Training Network
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Scientific Posters:
Role of intrinsically disordered regions in kinase signalling pathways: Substrate recognition in the c-Jun N-terminal kinase (JNK) pathway
XIIIe Journée Rhône-Alpes de RMN, Grenoble, France
June 2012