Hadas Raveh-Amit
Hadas Raveh-Amit graduated with excellence (Summa cum laude) from the Department of Microbiology and Immunology, Ben-Gurion University of the Negev, Beer Sheva, Israel. Her studies were supervised by Prof. Etta Livneh and focused on the translational control mechanism of Protein Kinase C family by regulatory elements located in the 5'-Untranslated Region. After receiving her Master, the candidate has continued her studies in the laboratory of Prof. Etta Livneh and is now at the final stage of her Phd. Hadas joined Biotalentum Ltd. located in Gödöllő, Hungary on August 2011 as a postdoctoral fellow.
| Researcher |
Hadas Raveh-Amit |
| Project Topic |
The role of the intrinsically disordered protein RYBP in neural and cardiac differentiation of pluripotent stem cells. |
| Project Description |
Protein disorder reaches high proportions in the proteomes of higher eukaryotes. In the human proteome, for example, about 12% of proteins are fully disordered, and 50% of proteins contain at least one long disordered region. The function of intrinsically disordered proteins (IDPs) is strongly associated with key cellular functions, such as signalling, regulation of the cell-cycle and transcription. In this study, we will focus on the role of IDPs in an embryonic stem (ES) cell based system. The RING1 and YY1 binding protein (RYBP) will be used as a model to study the role of this previously described IDP in ES differentiation. RYBP, a member of the polycomb group (PcG), is a transcriptional repressor and not surprisingly primarily localized in the nucleus. It was shown to interact with numerous partners and therefore form multiple complexes (such as with the BCOR complex, the MDM2 protein and ubiquitinated proteins). Interestingly, it was proposed that RYBP participates in a complex transcriptional regulatory network in mouse ES, however, its functions remain largely unknown. Therefore, we will examine the role of RYBP in neural and cardiac differentiation in an embryonic stem (ES) cell based system. For this purpose, we will use in vitro differentiation assays available at Biotalentum Ltd. Hopefully, these studies will open up important regulatory possibilities in neuronal lineage and cardiac development. |
| Project Home |
BioTalentum |
| Supervisor | Andras Dinnyes |
| Starting Date | 08/08/2011 |
| Publications |
Raveh-Amit H, Berzsenyi S, Vas V, Ye D, Dinnyes A, Resident stem cells and aging: ’til death do us part Accepted to Biogerontology: September 20, 2013 and published ahead of print DOI 10.1007/s10522-013-9469-9 Phetcharat P¥, Raveh-Amit H¥, Li T¥, Kitiyanan Y, Dinnyes A, Is aging a barrier to reprogramming? Lessons from induced pluripotent stem cells, (¥ equal contribution). Accepted to Biogerontology: August 16, 2013 and published ahead of print DOI 10.1007/s10522-013-9455-2 Shahaf G, Rotem-Dai N, Koifman G, Raveh-Amit H, Frost SA, Livneh E. PKCη is a negative regulator of AKT inhibiting the IGF-I induced proliferation. Exp Cell Res. 2012 Apr 15; 318:789-99.
INVITED LECTURE: Raveh-Amit H, Vas V, Berzsenyi S and Dinnyes A. Cancer stem cells networks and protein misfolding. The 8th European Congress of Biogerontology (ECB) - Healthy Ageing and Regenerative Medicine, March 10-13 2013 - Beer-Sheva - Dead Sea, Israel |
| Meetings |
FP7 funded workshop: Epigenetic and molecular influences on early development and stem cell differentiation, November 25, 2013 – Biotalentum Ltd, Godollo, Hungary Intensive Training Course: Intensive Training Module: Principles of NMR and NMR parameters used in IDP structure calculation, August 29-30, 2013 - CNRS-IBS, Grenoble, France FP7 funded workshop: Rabbit stem cell and their potential in creating models, March 26-27, 2013 - Biotalentum Ltd, Godollo, Hungary Intensive Training Module: SAXS and computational techniques, March 11-16, 2013 - EMBL, Hamburg, Germany Intensive Training Course: Scientific Writing and Project Management, October 25-28, 2012 - Tuscany, Italy FP7 funded workshop: Comparative embryonic stem cell research, September 25, 2012 - Biotalentum Ltd, Budapest, Hungary Training Course in Flow Cytometry Gallios-Navios-FC500 Application, May 21-25, 2012 - Beckman Coulter Inc, Nyon, Switzerland Advances in In-Vitro Cell and Tissue Culture Complementary Skills Training – Marie Curie Training Network Intensive Training Course 2011 – Marie Curie Training Network |
Scientific Posters:
Raveh-Amit H, Kobolak J, Ye D and Dinnyes A
"Nuclear transfer embryonic stem cells (NT-ESCs): new insights to perturbed maternal environment during periconceptional development due to assisted reproductive technologies"
Epigenetics and Periconception Environment conference, 24-25 April, 2013 - Antalya, Turkey
Dinnyes A, Berzsenyi S, Kovacs KA, Martinez Y, Ye D, Raveh-Amit H, and Nemes C
"Induced pluripotent stem cells to create cardiac, neuronal and smooth muscle tissue models"
Advances in In-Vitro Cell and Tissue Culture and 4th Annual Quasi-Vivo User Group Meeting, May 22-23, 2012 - Utrecht, The Netherlands
Hadas Raveh-Amit, Virag Vas, Sara Berzsenyi, Sara Santos-Franco, Andras Dinnyes
"Cancer Stem Cells and Intrinsic Disorder"
International Ilanit Conference in Eilat, Israel (10-13 February, 2014).