Intrinsically disordered proteins have been drawing more and more interest of the scientific community in the last decade. Due to extensive overlap in the NMR spectra of IDPs, the aim of the project is to develop new NMR pulse sequences specially tailored for IDP investigation, mainly focus on the 13C direct detection experiments.

Since more or less half of the higher eukaryote proteins contain at least one disordered region, this strategy is also vastly employed in the realm of viruses. Thus, we chose as the biologically relevant systems to study two viral IDPs involved in cancer developments, E7 oncoprotein from human Papillomavirus 16 and E1A protein from Adenovirus 2-5 hybrid.

Isabella C. Felli, Roberta Pierattelli

12th Chianti/INSTRUCT Workshop on BioNMR
Electron and Nuclear Relaxation for Structural Biology
June 2012 - Montecatini Terme (Pistoia), Italy
Poster: "NMR characterization of the intrinsically disordered N-terminal region of the E7 protein from Human Papillomavirus"

Intensive Training Module – Marie Curie Training Network
Protein Structure Analysis using NMR Chemical Shifts
May 2012 - Cambridge, United Kingdom

Complementary Skills Training – Marie Curie Training Network
Self Marketing, Project Management, Benchmarks and Competition in Research
February 2012 - St. Moritz, Switzerland


Intensive Training Module – Marie Curie Training Network
Structural and Unstructural Biology of Viral Proteins
January 2012 - Florence, Italy

Intensive Training Course 2011 – Marie Curie Training Network
Bioinformatics and Structural biology of IDPs
October 2011 - Budapest, Hungary