Tomáš Hošek


Tomáš Hošek graduated in Biophysics at the Masaryk University in Brno with thesis on plasma assisted deposition of photo-catalytic TiO2 films. He continued his studies at the same university with Master in Molecular Biophysics with thesis concerning the NMR and computational study of the interaction site of RNA-binding protein. During his master studies he participated in research project focused on drugs influencing RNA interference pathways at National Center for Biomolecular Research, where he acquired basic theoretical and practical skills in protein NMR spectroscopy. He has solid background in physics and molecular biology. He has been appointed by CERM group within the IDPbyNMR project.


Tomáš Hošek
Project Topic
Development of new NMR methods to study IDPs – Heteronuclear NMR
Project Description

Intrinsically disordered proteins have been drawing more and more interest of the scientific community in the last decade. Due to extensive overlap in the NMR spectra of IDPs, the aim of the project is to develop new NMR pulse sequences specially tailored for IDP investigation, mainly focus on the 13C direct detection experiments.

Since more or less half of the higher eukaryote proteins contain at least one disordered region, this strategy is also vastly employed in the realm of viruses. Thus, we chose as the biologically relevant systems to study two viral IDPs involved in cancer developments, E7 oncoprotein from human Papillomavirus 16 and E1A protein from Adenovirus 2-5 hybrid.

Project Home

Isabella C. Felli, Roberta Pierattelli

Starting Date 22/08/2011

Gil, Sergio, Tomáš Hošek, Zsofia Solyom, Rainer Kümmerle, Bernhard Brutscher, Roberta Pierattelli, and Isabella C Felli. 
“NMR Spectroscopic Studies of Intrinsically Disordered Proteins at Near-Physiological Conditions.” 
Angewandte Chemie 52(45): 11808–12. (2013)

Calçada, E.O., Felli, I.C., Hošek, T., and Pierattelli, R.
The heterogeneous structural behavior of E7 from HPV16 revealed by NMR spectroscopy.
ChemBioChem 14, 1876–1882. (2013)

12th Chianti/INSTRUCT Workshop on BioNMR
Electron and Nuclear Relaxation for Structural Biology
June 2012 - Montecatini Terme (Pistoia), Italy
Poster: "NMR characterization of the intrinsically disordered N-terminal region of the E7 protein from Human Papillomavirus"

Intensive Training Module – Marie Curie Training Network
Protein Structure Analysis using NMR Chemical Shifts
May 2012 - Cambridge, United Kingdom

Complementary Skills Training – Marie Curie Training Network
Self Marketing, Project Management, Benchmarks and Competition in Research
February 2012 - St. Moritz, Switzerland

Intensive Training Module – Marie Curie Training Network
Structural and Unstructural Biology of Viral Proteins
January 2012 - Florence, Italy

Intensive Training Course 2011 – Marie Curie Training Network

Bioinformatics and Structural biology of IDPs
October 2011 - Budapest, Hungary


Scientific Posters:



12th Chianti/INSTRUCT workshop on BioNMR, Montecatini Terme, Italy. 
June 17th-22th 2012