Intensive Training Course (S&T) 2012:
NMR methods for the study of structural order and disorder in proteins

Les Houches, September 09-14, 2012
Ecole de Physique des HouchesLes Houches, France

The course will focus on theoretical and practical aspects of modern multidimensional NMR spectroscopy and molecular modeling, to characterize Intrinsically Disordered Proteins (IDPs).


Report on the ITC    -   List of participants

This second IDPbyNMR training course will provide theoretical and practical tools for measurement and interpretation of experimental NMR parameters that are sensitive to both local and long-range conformational behavior of highly disordered states. This will cover the study of weak complexes that often characterize functional interactions involving IDPs, the measurement and interpretation of paramagnetic relaxation properties to detect transient interactions, and the combination of all of these parameters into self consistent ensemble descriptions. Reduced spectral resolution in the proton dimension and protein instability, that are characteristic of disordered states, will be addressed using fast multidimensional NMR methods such as projection and Hadamard spectroscopy, and SOFAST and BEST techniques, as well as 13C-direct detection approaches.

Topics of the talks:

  • Biomolecular NMR: A historical perspective
  • NMR sample preparation and isotope labeling
  • NMR pulse sequence design
  • NMR data acqusition and processing
  •  NMR hardware
  • Sequential NMR assignment strategies
  • Fast multidimensional NMR data acqusition
  • 13C detected NMR experiments
  • Paramagnetic NMR
  • NMR observables for highly disordered molecular systems
  • NMR methods for the study of protein dynamics
  • Structural ensembles from NMR chemical shifts
  • In-cell NMR
  • Chaperone activity of IDPs
  • NMR studies of the tau protein and other examples

Organizing Committe:
Bernhard Brutscher (CNRS-IBS)
Isabella C. Felli (CERM)
Roberta Pierattelli (CERM)

Up today the following scientists confirmed their participation (in alphabetic order):

Mikael Akke (University of Lund, Lund, Sweden)
d Bax (NIH-NIDDK, Bethesda, USA)
Bernhard Brutscher (CNRS-IBS, Grenoble, France)
Isabella C. Felli
(CERM, Florence, Italy)
Stephan Grzesiek (University of Basel, Basel, Switzerland)
Robert Konrat
(University of Wien, Wien, Austria)
Tania Kozyreva (Giotto Biotech, Sesto Fiorentino, Italy)
Brithe B. Kragelund (University of Copenhagen, Copenhagen, Denmark)
Rainer Kümmerle (Bruker BioSpin AG, Faellanden, Switzerland)
Ewen Lescop
(ICSN, Gif-Sur-Yvette, France)
Dominique Marion
(CNRS-IBS, Grenoble, France)
Roberta Pierattelli
(CERM, Florence, Italy)
Malene Ringkjobing-Jesen (CNRS-IBS, Grenoble, France)
Michael Sattler (CIPSM, Munich, Germany)
Philipp Selenko (FMP, Berlin, Germany)
Peter Tompa
(Vrije Universiteit Brussel, Brussel, Belgium)
Michele Vendruscolo
(University of Cambridge, Cambridge, United Kingdom)
Markus Zweckstetter
(Max Planck Institute, Göttingen, Germany)

A more detailed program is available here.

How to Apply

The max number of participants has been reached - REGISTRATION IS CLOSED!


Pictures from the event:

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